This regimen is FDA approved and supported by phase III INAVO120 for PIK3CA-mutant, HR-positive/HER2-negative advanced disease recurring on or after adjuvant endocrine therapy and without prior systemic therapy for advanced disease.
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ESR1 mutation
Acquired ESR1 mutation predicts relative resistance to further AI-based therapy and identifies patients for oral ER-targeting agents. Plasma ctDNA testing is widely used in practice.
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Elacestrant / Imlunestrant
Elacestrant is supported by phase III EMERALD and is established in ESR1-mutated disease after prior ET plus CDK4/6 inhibitor. Imlunestrant became FDA approved in 2025 for ESR1-mutated ER-positive/HER2-negative advanced disease after at least one line of endocrine therapy.
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T-DXd (HER2 IHC ultra-low/low)
DESTINY-Breast06 and FDA approval moved trastuzumab deruxtecan earlier for HR-positive HER2-low/HER2-ultralow disease after progression on endocrine therapy in the metastatic setting.
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Chemo
Guidelines continue to support single-agent chemotherapy in later lines and in patients no longer suited to endocrine-based strategies.
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At least 2 prior lines of systemic rx
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PIK3CA mutation
PIK3CA mutation supports PI3K-pathway targeting. It is relevant both for alpelisib-based therapy after endocrine progression and for inavolisib in the earlier, narrowly defined endocrine-resistant first metastatic-line setting.
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PIK3CA / AKT1 / PTEN alteration
CAPItello-291 and FDA labeling define PIK3CA, AKT1, or PTEN alteration as the key biomarker group for capivasertib plus fulvestrant.
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gBRCA mutation
Pathogenic germline BRCA1/2 mutation supports use of olaparib or talazoparib in HER2-negative metastatic breast cancer, generally after appropriate endocrine use in HR-positive disease or when endocrine therapy is no longer appropriate.
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Everolimus + Exemestane OR Fulvestrant-based Therapy +/- CDK4/6 Inhibitor
BOLERO-2 firmly supports everolimus-exemestane after prior AI exposure. For post-CDK4/6 continuation/switch, evidence exists for selected regimens such as abemaciclib-fulvestrant and ribociclib with endocrine switch, but this remains less standardized than biomarker-directed branches.
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T-DXd (HER2 IHC ultra-low/low)
DESTINY-Breast06 and FDA approval moved trastuzumab deruxtecan earlier for HR-positive HER2-low/HER2-ultralow disease after progression on endocrine therapy in the metastatic setting.
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Chemo
Guidelines continue to support single-agent chemotherapy in later lines and in patients no longer suited to endocrine-based strategies.
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At least 2 prior lines of systemic rx